Occurrence of a Phosphate Ester of a Hydroxyamino-Acid

DURING a recent investigation of the dialysate of calf embryo muscle juice by means of paper chromatography, a very slow-moving spot has been noticed which does not appear to correspond with any substance previously recognized in such extracts. Fractionation of the dialysate on chromatograms of amberlite I.R.

4 indicated that it was due to a weakly acidic substance. It was eluted from these chromatograms in the same fraction as phosphoryl amino-ethanol, which, contrary to the finding of Outhouse1, appears to be present in calf embryos. Thus it seemed worth investigating whether the unknown substance might be another phosphoric ester.

This was done as follows.

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